Electronic Theses and Dissertation

Enzim merupakan kumpulan daripada protein yang memiliki peran penting dalam aktivitas biologis. salah satu enzim yang berperan penting ialah enzim kitinase. penelitian ini bertujuan untuk mengukur aktivitas enzim kitinase dari jamur kitinolitik asal limbah eksoskeleton kepiting bakau (scylla serrata) dan rajungan bintang (portunus sanguinolentus). pendekatan penelitian yang digunakan adalah kuantitatif dengan metode observasi. parameter yang diamati ialah aktivitas enzim kitinase fungi kitinolitik berdasarkan variasi ph. aktivitas enzimatik ditentukan dengan mengukur kekeruhan koloid kitin pada serapan panjang gelombang 660 nm menggunakan spektrofotometer. data dianalisis secara deskriptif dan ditampilkan secara grafis. berdasarkan hasil penelitian yang dilakukan, ph optimal setiap isolat dalam menghasilkan enzim kitinase ditemukan berada pada kisaran netral, yaitu ph 6 sampai 8. kata kunci : kitinase, kitinolitik, ph

Enzymes are a collection of proteins that have an important role in biological activity. One of the enzymes that plays an important role is the chitinase enzyme. This study aims to measure the activity of the chitinase enzyme from chitinolytic fungi from exoskeleton waste of Mud Crab (Scylla serrata) and Star crab (Portunus Sanguinolentus). The research approach used is quantitative with the observation method. Parameters observed were chitinolytic fungi chitinase enzyme activity based on variations in pH. Enzymatic activity was determined by measuring the turbidity of colloidal chitin at an absorption wavelength of 660 nm using a spectrophotometer. Data were analyzed descriptively and displayed graphically. Based on the results of the research conducted, the optimal pH for each isolate to produce chitinase enzymes was found to be in the neutral range, namely pH 6 to 8. Keywords: chitinase, chitinolytic, pH