Electronic Theses and Dissertation

Salmonella sp. merupakan salah satu patogen yang sering kali menyebabkan infeksi pada unggas. Sumber patogen dapat berasal dari pakan, air dan bahkan kotoran unggas itu sendiri yang dapat menyebar melalui udara. Penelitian ini bertujuan untuk mengisolasi bakteri Salmonella sp. dari cemaran udara pada kandang ayam pedaging tipe kandang close house di Peternakan Japfa Ie Suum, Aceh Besar. Penelitian dilakukan secara cross seccional, dan pengambilan sampel cemaran udara dilakukan sebanyak 2 kali yaitu pada pagi dan sore hari. Pengambilan pertama dilakukan pada jam 10.00 WIB dengan cara meletakkan plate media SSA dan dibuka selama 15 menit dengan ketinggian yang bervariasi mulai dari 5 cm, 10 cm, dan 15 cm dari lantai kandang dan jarak tiap plate 12 m, 24 m, 36 m dari exhaust fan. Pengambilan sampel kedua dilakukan pada jam 16.00 WIB dengan variasi ketinggian dan jarak yang sama. Plate agar selanjutnya dibawa ke Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala dan di inkubasi selama 24 jam dengan suhu 37˚C. Identifikasi bakteri dilakukan melalui pengamatan morfologi koloni, pewarnaan Gram, dan uji biokimia. Hasil isolasi bakteri dari kandang ayam close house dapat diketahui bahwa terdapat cemaran bakteri Salmonella sp. pada kandang ayam broiler tipe kandang close house di Peternakan Japfa Ie Suum, Aceh Besar. Cemaran udara yang paling tinggi terjadi pada ketinggian 5 cm. Pencemaran udara paling tinggi juga diamati pada pengambilan sampel sore hari. Berdasarkan hasil penelitian dapat disimpulkan bahwa Salmonella sp. dapat di isolasi dari udara kandang ayam pedaging tipe close house dan terdapat beberapa jenis Salmonella yang ditemukan yaitu Salmonella gallinarum, Salmonella paratyphi, Salmonella typhimurium.

Kata kunci: Ayam pedaging, cemaran udara, close house, kandang, Salmonella

Salmonella sp. is one of the pathogens that often causes infection in poultry. Sources of pathogens can come from feed, air, and even poultry manure itself which can be spread through the air. This study aims to isolate the bacteria Salmonella sp. from air pollution in a close house type broiler cage at Japfa Ie Suum Ranch, Aceh Besar. The study was conducted in a cross sectional manner, and sampling was carried out 2 times, namely in the morning and evening. The first collection was carried out at 10.00 WIB by placing the SSA media plate and opening it for 15 minutes with varying differences ranging from 5 cm, 10 cm, and 15 cm from the cage floor and the distance between each plate was 12 m, 24 m, 36 m exhaust fan. The second sampling was carried out at 16.00 WIB with the same variation in height and distance. The agar plate was then taken to the Microbiology Laboratory of the Faculty of Veterinary Medicine, Syiah Kuala University and stored for 24 hours at a temperature of 37˚C. Bacterial identification was carried out by observing colonies, Gram staining, and biochemical tests. The results of bacterial isolation from close house chicken coops showed that there was contamination of Salmonella sp. in a close house broiler chicken coop at Japfa Ie Suum Ranch, Aceh Besar. The highest air pollution occurs at a height of 5 cm. The highest air pollution was also observed in the afternoon sampling. Based on the results of the study it can be said that Salmonella sp. can be isolated from the air of a close house broiler cage and there are several types of Salmonella found, namely Salmonella gallinarum, Salmonella paratyphi, Salmonella typhimurium. Keywords: Broiler, air pollution, close house, cage, Salmonella