Electronic Theses and Dissertation

Telah dilakukan uji total phenolic (TPC) dan total flavonoid (TFC) serta uji antioksidan ekstrak etanol daun kitolod (Hippobroma Longiflora L.) asal Aceh. Hasil uji fitokimia menunjukkan bahwa ekstrak etanol daun kitolod mengandung senyawa alkaloid, steroid, saponin, phenolic dan flavonoid. Analisis kuantitatif senyawa total phenolic sampel dilakukan menggunakan reagen Folin-Ciocalteu dan dianalisis dengan menggunakan alat spektrofotometer UV-Vis pada panjang gelombang 762 nm. Hasil memperlihatkan bahwa ekstrak etil asetat, kloroform dan n-butanol hasil partisi dari esktrak etanol daun kitolod memiliki kadar senyawa total phenolic berturut-turut sebesar 23,89; 35,24 dan 139,21 mg (GAE/g). Analisis kuantitatif senyawa total flavonoid sampel dilakukan dengan menggunakan kuersetin sebagai pembanding. Hasil memperlihatkan bahwa kadar senyawa total flavonoid ekstrak etil asetat, kloroform dan n-butanol hasil partisi dari ekstrak etanol daun kitolod yang diukur pada panjang gelombang 440 nm adalah berturut-turut sebesar 4,82; 7,71 dan 11,48 mg (QE/g). Hasil aktivitas antioksidan ekstrak n-butanol hasil partisi dari ekstrak etanol daun kitolod dilakukan dengan menggunakan metode DPPH, dan hasil analisis menunjukkan bahwa ekstrak n-butanol hasil partisi dari ekstrak etanol daun kitolod memiliki nilai IC50 sebesar 9,117 ppm.

Kata Kunci: Daun kitolod (Hippobroma Longiflora L), senyawa total phenolic, senyawa total flavonoid, Antioksidan

Total phenolic (TPC) and total flavonoid (TFC) tests have been carried out as well as antioxidant tests for the ethanol extract of kitolod (Hippobroma Longiflora L.) leaves from Aceh. Phytochemical test results showed that the ethanol extract of kitolod leaves contained alkaloids, steroids, saponins, phenolics and flavonoids. Quantitative analysis of the total phenolic compounds in the sample was carried out using Folin-Ciocalteu reagent and analyzed using a UV-Vis spectrophotometer at a wavelength of 762 nm. The results showed that the ethyl acetate, chloroform and n-butanol extracts from the ethanol extract of kitolod leaves had total phenolic compounds content of 23,89; 35,24 and 139,21 mg (GAE/g). Quantitative analysis of the total flavonoid compounds in the sample was carried out using quercetin as a comparison. The results showed that the total flavonoid content of the ethyl acetate, chloroform and n-butanol extracts from the ethanol extract of kitolod leaves measured at a wavelength of 440 nm were 4,82; 7,71 and 11,48 mg, respectively. (QE/g). The results of the antioxidant activity of the partitioned n-butanol extract from the ethanol extract of kitolod leaves were carried out using the DPPH method, and the results of the analysis showed that the partitioned n-butanol extract from the ethanolic extract of kitolod leaves had an IC50 value of 9,117 ppm Keywords: Kitolod leaves (Hippobroma Longiflora L), total phenolic compounds, total flavonoid compounds, antioxidants