Electronic Theses and Dissertation

Madu telah dimanfaatkan sebagai bahan obat untuk mengobati penyakit infeksi. Salah satu lebah penghasil madu yaitu Heterotrigona itama atau kelulut. Penelitian ini bertujuan untuk mengetahui kualitas dan aktivitas antimikroba madu kelulut terhadap bakteri Staphylococcus aureus, Escherichia coli, dan jamur Candida albicans. Parameter kualitas madu yang diukur meliputi uji organoleptik, nilai aktivitas enzim diastase, kadar hidroksimetilfurfural (HMF), kadar air, kadar gula pereduksi, keasaman madu, dan cemaran logam timbal (Pb), dengan prosedur uji berdasarkan Standar Nasional Indonesia (SNI) 8664:2018 tentang madu. Uji aktivitas antimikroba madu kelulut menggunakan metode difusi sumuran dengan variasi konsentrasi (b/v) 5; 10; 15; 20 dan 30%. Hasil penelitian diperoleh empat parameter kualitas madu yang memenuhi standar SNI yaitu organoleptik, kadar hidroksimetilfurfural (HMF) sebesar 0,0054 mg/ kg, kadar air sebesar 27,41%, dan kadar cemaran logam timbal (Pb) sebesar 0,001198 mg/kg. Hasil uji aktivitas antimikroba menunjukkan madu kelulut dapat menghambat bakteri Staphylococcus aureus dan Escherichia coli pada konsentrasi terkecil 5% dengan diameter zona hambat sebesar 14,7 mm dan 9,3 mm serta diameter zona hambat terbesar sebesar 35,8 mm dan 25 mm pada konsentrasi 30%, sedangkan uji aktivitas antimikroba terhadap jamur Candida albicans tidak menghasilkan zona hambat.

Honey has been used as a medicinal material to treat infectious diseases. One of the honey-producing bees is Heterotrigona itama or kelulut. This study aimed to determine the quality and antimicrobial activity of kelulut honey against Staphylococcus aureus and Escherichia coli bacteria, also Candida albicans fungi. The quality of honey parameters which is measured i.e., organoleptic, activity value of the enzyme diastase, hydroxymethylfurfural (HMF) content, moisture content, reducing sugar level, the acidity of honey, and lead metal (Pb) contamination content, with a test procedure based on the Indonesian National Standard (SNI) 8664: 2018 on honey. Antimicrobial activity test of kelulut honey using the good diffusion method with a concentration variation (w/v) of 5; 10; 15; 20 and 30%. The results of the study obtained four honey quality parameters that meet SNI standards, namely organoleptic, hydroxymethylfurfural (HMF) content of 0.0054 mg/kg, moisture content of 27.41%, and lead metal (Pb) contamination content of 0.001198 mg/kg. The results of the antimicrobial activity test showed that kelulut honey can inhibit Staphylococcus aureus and Escherichia coli bacteria at the smallest concentration of 5% with inhibitory zone diameter of 14.7 mm and 9.3 mm and the largest inhibitory zone diameter of 35.8 mm and 25 mm at a concentration of 30%, while the antimicrobial activity test against Candida albicans fungi did not produce an inhibition zone.