Electronic Theses and Dissertation

Askariasis adalah penyakit infeksi yang disebabkan oleh cacing Ascaris lumbricoides. Infeksi cacing di Indonesia paling banyak disebabkan oleh Soil Transmitted Helminths (STH). Kirinyuh merupakan salah satu tumbuhan yang memiliki aktivitas anti helminth. Adapun tujuan penelitian ini yaitu menentukan kandungan total fenolik, tanin dan flavonoid serta menentukan aktivitas ekstrak metanol daun Kirinyuh sebagai anti helminth terhadap Ascaridia galli. Kadar total fenolik, tanin dan flavonoid masing-masing didapatkan sebesar 30,34 mg GAE/g, 187,76 mg AT/g dan 91,25 mg QE/g. Ascaridia galli digunakan sebagai organisme uji dalam penelitian ini karena memiliki persamaan morfologi dengan cacing Ascaris lumbricoides. Organisme uji dibagi ke dalam enam kelompok uji, dengan tiap kelompok uji diberikan 30 ekor cacing. Kelompok uji yang digunakan yaitu ekstrak metanol daun Kirinyuh pada konsentrasi 5, 10, 15 dan 20%, serta kontrol negatif yaitu NaCl 0,9% dan kontrol positif berupa piperazin 1,5%. Jumlah kematian dan persentase kematian cacing selama 3 jam tiap 10 menit dikumpulkan sebagai data hasil uji anti helminth. Berdasarkan hasil penelitian didapatkan bahwa pada tiap konsentrasi ekstrak metanol daun Kirinyuh memiliki efek anti helminth. Efek anti helminth paling baik didapatkan pada konsentrasi 20% yang mampu menyebabkan 100% kematian pada cacing, dengan nilai LC50 sebesar 8,98%.
Kata kunci : Kirinyuh, lisis, A. galli, anti helminth.

Ascariasis is an infectious disease caused by Ascaris lumbricoides. Worm infections in Indonesia are mostly caused by Soil Transmitted Helminths (STH). Siam weed is one of a plant that has anti-helminth activity. The aims of this study were to determine the total compound of phenolic, tannin, and flavonoid content, and also to determine the anti-helminth activity from methanolic extract of Siam weed leaves against Ascaridia galli. The total phenolic, tannin and flavonoid levels were found consecutively at 30,34 mg GAE/g, 187,76 mg AT/g and 91,25 mg QE/g. Ascaridia galli was used as the experimental organism in this study, because it has morphological similarities from Ascaris lumbricoides. The experimental organism were divided into six test groups, with 30 worm in each test group. The test groups used was methanolic extract of Siam weed leaves at concentration of 5,10,15,and 20%, as well as the negative control which was 0,9% NaCl, and the positive control which was 1,5% piperazine. The number of deaths and the percentage of worm for 3 hours every 10 minutes were collected as data on the results of anti-helminth test. Based on the result, it was found that at each concentration of the methanolic extract of Siam weed leaves it had an anti-helminth effect. The best effect of anti-helminth is obtained at 20% concentration which can cause 100% death in worms, with 8,98% value of LC50. Keyword : Kirinyuh, lisis, A. galli, anti helminth