Electronic Theses and Dissertation

Pengendalian vektor nyamuk Culex sp. menggunakan larvasida sintetik terus-menerus berdampak terhadap resistensi vektor sehingga diperlukan biolarvasida untuk mengurangi dampak tersebut. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daun Tagetes erecta L. terhadap kematian larva nyamuk Culex sp, nilai LC50, dan tingkah laku larva nyamuk Culex sp.. Pendekatan penelitian adalah kuantitatif dengan jenis penelitian eksperimental menggunakan Rancangan Acak Lengkap Faktorial yang terdiri dari faktor A (jenis pelarut) 2 taraf dan faktor B (jenis konsentrasi) 6 taraf termasuk kontrol negatif dan kontrol positif sehingga didapatkan 12 kombinasi perlakuan dan 3 ulangan. Parameter penelitian adalah kematian larva nyamuk Culex sp. instar III, nilai LC50, dan tingkah laku larva nyamuk Culex sp.. Data dianalisis dengan ANAVA dengan nilai Koefisien Keragaman 4% dilakukan uji lanjut Beda Nyata Jujur. Nilai LC50 didapatkan dengan analisis probit. Hasil ANAVA kombinasi perlakuan faktor A dan B menunjukkan hasil sangat berbeda nyata (Fhitung(11,66) ≥ Ftabel(3,90)) terhadap kematian larva nyamuk Culex sp. Analisis probit menunjukkan nilai LC50 adalah 562,34 ppm (pelarut etanol) dan 978,26 ppm (pelarut air). Pengamatan tingkah laku larva menunjukkan pergerakan larva dari aktif menjadi tidak aktif dengan posisi larva di dasar wadah. Simpulan dari penelitian adalah kombinasi jenis pelarut dan jenis konsentrasi ekstrak daun Tagetes erecta L. berpengaruh terhadap kematian larva nyamuk Culex sp. dengan nilai LC50 562,34 ppm (pelarut etanol), 978,26 ppm (pelarut air), dan tingkah laku larva saat pemaparan menunjukkan penurunan intensitas gerakan dengan posisi larva berada di dasar wadah.
Kata kunci: Biolarvasida, Larva instar III Culex sp., Tagetes erecta L.

Culex sp. mosquito vector control using synthetic larvicides continuously has an impact on vector resistance so that biolarvicides are needed to reduce this impact. This study aims to determine the effect of Tagetes erecta L. leaf extract on the death of Culex sp mosquito larvae, LC50 values, and the behavior of Culex sp mosquito larvae. The research approach was quantitative with this type of experimental research using a Completely Randomized Factorial Design consisting of factor A. (type of solvent) 2 levels and factor B (type of concentration) 6 levels including negative control and positive control so that 12 treatment combinations and 3 replications were obtained. The research parameter was the mortality of Culex sp. mosquito larvae. instar III, LC50 value, and behavior of Culex sp. mosquito larvae. The data were analyzed by ANOVA with a Coefficient of Diversity value of 4%, followed by an Honest Significant Difference test. The LC50 value was obtained by probit analysis. The ANOVA results of the combined treatment of factors A and B showed significantly different results (Fcount(11.66) ≥ Ftable(3.90)) on the mortality of Culex sp. mosquito larvae. Probit analysis showed the LC50 values were 562.34 ppm (ethanol solvent) and 978.26 ppm (water solvent). Observation of larval behavior showed the movement of the larvae from active to inactive with the position of the larvae at the bottom of the container. The conclusion of the study is that the combination of the type of solvent and the type of concentration of Tagetes erecta L. leaf extract has an effect on the mortality of Culex sp. mosquito larvae. Keyword: Biolarvacide, III instar larva Culex sp., Tagetes erecta L