Electronic Theses and Dissertation

Kenaikan hormon kortisol berkorelasi dengan peningkatan aktivitas adrenal fetus.
Kelenjar adrenal fetus membesar, dan menjadi lebih responsif terhadap stimulasi hormon
adrenokortikotropik (ACTH) yang merupakan sinyal endokrin yang memulai proses kelahiran..
Ketika ACTH disekresi oleh kelenjar pituitari, hormon ini mengikuti aliran darah dan mencapai
kelenjar adrenal, yang berada di atas ginjal, dan memicu sekresi hormon kortisol. Penelitian ini
bertujuan untuk mengetahui kadar hormon kortisol pada kuda gayo saat menjelang partus.
Penelitian ini adalah penelitian eksploratif terhadap kadar hormon kortisol pada kuda gayo betina
menjelang partus yang di pelihara secara tradisional dan menggunakan sampel plasma darah untuk
melihat konsentrasi hormon kortisol menggunakan metode enzyme linked immunosorbent assay
(ELISA). Tiga ekor kuda gayo yang sedang bunting 11 bulan diambil darahnya sekali seminggu
selama 4 minggu saat menjelang partus. Konsentrasi hormon kortisol diukur dengan competitive
ELISA menggunakan DRG kortisol (DRG instruments GmbH, Germany). Hasil pengukuran
terhadap kadar hormon kortisol pada 3 ekor kuda gayo betina satu bulan menjelang partus dapat
diketahui bahwa kadar hormon kortisol tertinggi terjadi pada 1 minggu menjelang partus (H-8)
yaitu 176,36±28,27 ng/ml dengan kisaran 152,23 - 207,47 ng/ml. Sementara 1 hari menjelang
partus terjadi penurunan hormon kortisol dengan rataan 144,98±68,24 ng/ml dengan kisaran 70,15
- 203,79 ng/ml. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa adanya peningkatan
kadar hormon kortisol pada H-8 yang dapat menjadi indikator waktu partus pada kuda gayo.
Kata kunci: Kuda gayo, kortisol, ELISA, partus.

An increase in the hormone cortisol correlates with an increase in fetal adrenal activity. The adrenal glands of the fetus enlarge, and become more responsive to the stimulation of adrenocorticotropic hormone (ACTH), which is the endocrine signal that initiates the birth process. When ACTH is secreted by the pituitary gland, it travels into the bloodstream and reaches the adrenal glands, which are on top of the kidneys, and triggers the secretion of the hormone cortisol. This study aims to determine the levels of the hormone cortisol in Gayo horses just before parturition. This research is an exploratory study of cortisol hormone levels in Gayo horses before parturition which are traditionally reared and uses blood plasma samples to see cortisol hormone concentrations using the enzyme linked immunosorbent assay (ELISA) method. Three Gayo horses who were 11 months pregnant had their blood drawn once a week for 4 weeks just before parturition. Cortisol hormone concentration was measured by competitive ELISA using DRG cortisol (DRG instruments GmbH, Germany). The results of measurements of cortisol hormone levels in 3 female Gayo horses one month before parturition showed that the highest cortisol hormone levels occurred 1 week before parturition (D-8), namely 176.36±28.27 ng/ml with a range of 152.23 - 207.47 ng/ml. While 1 day before parturition there was a decrease in the hormone cortisol with an average of 144.98±68.24 ng/ml with a range of 70.15 - 203.79 ng/ml. Based on the results of this study it can be concluded that there is an increase in cortisol hormone levels on D-8 which can be an indicator of parturition time in Gayo horses. Keywords: Gayo horse, cortisol, ELISA, parturition.