Electronic Theses and Dissertation

Aquilaria malaccensis merupakan salah satu tumbuhan obat yang mempunyai aktivitas sebagai antijamur. Penelitian ini telah dilakukan untuk mengetahui aktivitas antijamur ekstrak n-heksana, etil asetat dan etanol daun A. malaccensis dalam menghambat pertumbuhan jamur Candida albicans. Ekstraksi daun A. malaccensis menggunakan metode maserasi bertingkat. Terhadap ekstrak dilakukan karakterisasi, uji fitokimia, analisis GC-MS dan uji aktivitas antijamur menggunakan metode sumuran. Variasi konsentrasi ekstrak yaitu 5; 10; 15; 20; dan 30%. Simplisia daun A. malaccensis mengandung kadar air sebesar 7,8%. Ekstrak n-heksana daun A. malaccensis mengandung kadar air sebesar 18,33%. Ekstrak etil asetat daun A. malaccensis mengandung kadar air sebesar 24,33%, kadar sari larut air 2,64%, kadar sari larut etanol 56,26% dan kadar abu total 2,06%. Ekstrak etanol daun A. malaccensis mengandung kadar air sebesar 13,33%, kadar sari larut air 25,83%, kadar sari larut etanol 73,33% dan kadar abu total 9%. Analisis uji fitokimia menunjukkan bahwa simplisia ekstrak etil asetat dan etanol daun A. malaccensis mengandung senyawa golongan flavonoid, alkaloid, saponin, tannin dan steroid. Ekstrak n-heksana daun A. malaccensis mengandung senyawa golongan steroid. Analisis GC-MS ekstrak etil asetat mengandung senyawa Hexadecanoic acid, methyl ester (14,78%), ß-Sitosterol (10,93%), dan Neophytadiene (10,11%). Analisis GC-MS ekstrak etanol mengandung senyawa 9-Octadecenoic acid (Z)-, methyl ester (32,79%), Cyclohexane, 1,3,5-triphenyl- (20,13%) dan Hexadecanoic acid,methyl ester (10,17%). Ekstrak n-heksana daun A. malaccensis tidak menunjukkan aktivitas antijamur terhadap C. albicans. Ekstrak etil asetat daun A. malaccensis dengan konsentrasi 5; 10; 15; 20; dan 30% pada jamur C. albicans memiliki diameter zona hambat berturut-turut sebesar 7,03; 9,00; 10,83; 13,70; dan 16,93 mm sedangkan pada ekstrak etanol daun A. malaccensis hambat berturut-turut sebesar 6,13; 7,53; 8,60; 9,10; dan 10,61 mm.

Kata kunci: Aquilaria malaccensis, Daun Gaharu, Candida albicans, Maserasi bertingkat

Aquilaria malaccensis is one of the herbs that have antifungal activity. This study has been conducted to determine the antifungal activity of n-hexane, ethyl acetate and ethanol A. malaccensis leaves extracts against Candida albicans. The method that was used in this study was A. malaccensis leaves extraction using stage maceration. A. malaccensis leaves extracts were evaluated for characterization of extract, phytochemical test, GC-MS analysis, and antifungal activity by well diffusion method. The concentration of the extract was 5; 10; 15; 20; and 30%. A. malaccensis leaves simplicial characterization contains water content value of 7,8%. n-Hexane extract from A. malaccensis leaves contains a water content of 18,33%. Ethyl Acetate extract from A. malaccensis leaves contains a water content value of 24,33%, water-soluble extract content of 2,64%, ethanol-soluble extract content of 56,26%, and total ash content of 2,06%. Ethanol extract from A. malaccensis leaves contains a water content value of 13,33%, water-soluble extract content of 25,83%, ethanol-soluble extract content of 73,33%, and total ash content of 9%. The phytochemical analysis showed that A. malaccensis leaves simplicial ethyl acetate and ethanol extract contained flavonoid, alkaloid compounds, saponins, tannins, steroids and n-hexane contains steroids. GC-MS analysis of ethyl acetate extract contains compounds Hexadecanoic acid, methyl ester (14,78%), ß-Sitosterol (10,93%), and Neophytadiene (10,11%). GC-MS analysis of ethanol extract contains 9-Octadecenoic acid (Z)-, methyl ester (32,79%), Cyclohexane, 1,3,5-triphenyl- (20,13%), and Hexadecanoic acid, methyl ester (10,17%). The results of antifungal activity showed that A. malaccensis leaves n-hexane extract exhibited no activity against C. albicans, but A. malaccensis leaves ethyl acetate extract with a concentration of 5; 10; 15; 20 and 30% against C. albicans shown inhibition zone by 7,03; 9,00; 10,83; 13,70; and 16,93 mm respectively and A. malaccensis leaves ethanol extract against C. albicans shown inhibition zone by 10,37; 10,44; 11,63; 12,21; and 13,34 mm respectively. Keywords : Aquilaria malaccensis, Agarwood leaves, Candida albicans, Stage maceration