Electronic Theses and Dissertation

Penelitian ini bertujuan mengetahui gambaran histologis dan perkembangan folikel ovarium sapi aceh yang ditransplantasikan pada kelinci lokal bunting semu. Penelitian ini menggunakan sembilan ekor kelinci berumur 3-5 tahun, bobot badan 1,5-2,9 kg. Setelah adaptasi selama 30 hari, seluruh kelinci dibagi dalam tiga kelompok perlakuan yaitu, K1 (n=3) kelompok kelinci yang dilakukan transplantasi ovarium sapi aceh selama 3 hari, K2 (n=3) kelompok kelinci yang dilakukan transplantasi ovarium sapi aceh selama 5 hari, dan K3 (n=3) kelompok kelinci yang dilakukan transplantasi ovarium sapi aceh selama 7 hari. Induksi bunting semu dilakukan dengan menggunakan PMSG dan hCG. Kelinci diinjeksi dengan 100 IU PMSG secara intramuskulus dan diikuti tiga hari kemudian dengan injeksi 75 IU hCG secara intravena. Transplantasi ovarium sapi aceh dilakukan pada hari ke-8 (hari ke-0 adalah hari ketika injeksi hCG). Jumlah folikel dan perubahan histologis folikel ovarium sapi aceh ditentukan melalui pengamatan histologis dengan pewarnaan HE. Perkembangan folikel ovarium utuh masih dapat dilihat pada semua kelompok perlakuan meskipun ditemukan beberapa gambaran histopatologis. Jumlah folikel tersier pada K1; K2; dan K3 masing-masing adalah 24,67±7,37; 20,67±7,57; dan 9,67±3,79 (P

ABSTRACT This study aims to determine the histological features and ovarian follicle development of aceh cattle transplanted into pseudo-pregnant local rabbits. This study used 9 rabbits aged 3-5 years, body weight 1.5-2.9 kg. After adaptation for 30 days, all rabbits were divided into three treatment groups, namely, K1 (n=3) group of rabbits that underwent an ovary transplant from aceh cattle for 3 days, K2 (n=3) group of rabbits that underwent ovary transplantation from aceh cattle for 5 days, and K3 (n = 3) group of rabbits that underwent ovarian transplantation from aceh cattle for 7 days. Pseudo-pregnancy induction was carried out using PMSG and hCG. Rabbits were injected with 100 IU of PMSG intramuscularly and followed three days later by intravenous injection of 75 IU of hCG. Acehnese cattle ovary transplantation was carried out on the 8th day (day 0 was the day when the hCG was injected). The number of follicles and histological changes in ovarian follicles in aceh cattle were determined by histological observation with HE staining. The development of intact ovarian follicles could still be seen in all treatment groups although some histopathological features were found. Number of tertiary follicles on K1; K2; and K3 respectively 24.67 ± 7.37; 20.67±7.57; and 9.67±3.79 (P