Electronic Theses and Dissertation

Telah dilakukan penelitian tentang uji aktivitas sitotoksik ekstrak etil asetat bunga biduri (Calotropis gigantea) dengan metode brine shrimp lethality test (BSLT) pada bulan Februari – Maret 2023 di Laboratorium Kimia Laut dan Bioteknologi Perikanan, Fakultas Kelautan dan Perikanan, Universitas Syiah Kuala. Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder yang terdapat pada bunga biduri (C. gigantea) menggunakan metode maserasi dan menentukan aktivitas sitotoksik pada ekstrak etil asetat bunga biduri (C. gigantea). Hasil senyawa metabolit sekunder yang diperoleh dari ekstrak etil asetat bunga biduri (C. gigantea) adalah flavonoid, alkaloid, tanin, dan glikosida. Hasil uji sitotoksik terhadap larva udang Artemia salina Leach dengan metode BSLT dilakukan pada beberapa variasi konsentrasi yaitu 2000 ppm, 1000 ppm, 500 ppm, 250 ppm, 100 ppm, dan kontrol. Berdasarkan hasil pengamatan dan analisis data yang diperoleh bahwa ekstrak etil asetat bunga biduri (C. gigantea) memiliki potensi sitotoksik dengan nilai LC50 sebesar 189.883 ppm yang termasuk kategori moderat/cukup aktif
Kata Kunci : Bunga biduri (Calotropis gigantea), Sitotoksik, BSLT, Ekstraksi

Cytotoxic activity test of ethyl acetate extract of biduri flower (Calotropis gigantea) with brine shrimp lethality test (BSLT) method was conducted in February - March 2023 at Marine Chemistry and Fisheries Biotechnology Laboratory, Faculty of Marine and Fisheries, University Syiah Kuala. This study aims to determine the content of secondary metabolites contained in biduri flower (C. gigantea) using maceration method and determine the cytotoxic activity of ethyl acetate extract of biduri flower (C. gigantea). The results of secondary metabolite compounds obtained from ethyl acetate extract of biduri flower (C. gigantea) are flavonoids, alkaloids, tannins, and glycosides. The results of cytotoxic tests on Artemia salina Leach shrimp larvae using the BSLT method were carried out at several concentration variations, namely 2000 ppm, 1000 ppm, 500 ppm, 250 ppm, 100 ppm, and control. Based on the results of observations and data analysis obtained that the ethyl acetate extract of biduri flower (C. gigantea) has cytotoxic potential with an LC50 value of 189.883 ppm which is included in the moderate/quite active category Keywords: Biduri flower (Calotropis gigantea), Cytotoxic, BSLT, Extraction