Electronic Theses and Dissertation

Minyak atsiri serai wangi diketahui memiliki aktivitas antibakteri, salah satunya terhadap bakteri Streptococcus mutans. Bakteri S. mutans merupakan penyebab utama terjadinya karies gigi yang terjadi pada jaringan keras gigi. Penelitian ini bertujuan untuk mengetahui aktivitas minyak atsiri serai wangi dalam menghambat pertumbuhan bakteri S. mutans isolat klinis. Metode uji aktivitas antibakteri yang digunakan pada penelitian ini yaitu metode sumuran. Hasil pengujian karakterisasi minyak atsiri serai wangi yaitu berwarna kuning muda, berbau khas minyak atsiri serai wangi, bobot jenis 0,879 g/mL, indeks bias 1,471, dan kelarutan dalam etanol 80% sebesar 1:2. Analisis GC-MS minyak atsiri serai wangi diperoleh 20 kandungan senyawa dengan komponen utama sitronelal 45,83%, geraniol 5,88%, dan sitronelol 15,55%. Uji aktivitas antibakteri minyak atsiri serai wangi terhadap S. mutans isolat klinis menunjukkan bahwa minyak atsiri serai wangi dengan variasi konsentrasi 2,5; 5; 10; 20; 30% memiliki zona hambat berturut-turut sebesar 9,43; 11,8; 13,3; 14,6; dan 16,8 mm. Hasil penelitian ini menunjukkan bahwa minyak atsiri serai wangi (Cymbopogon nardus L.) berpotensi dalam menghambat pertumbuhan S. mutans isolat klinis.
Kata kunci: aktivitas antibakteri, Streptococcus mutans isolat klinis, minyak atsiri serai wangi, Cymbopogon nardus L.

Citronella essential oil is known to have antibacterial activity against Streptococcus mutans, the main cause of dental caries that affects the hard tissue of the teeth. This study aims to determine the activity of citronella essential oil in inhibiting the growth of clinical isolates of S. mutans. The agar well diffusion method was used as the antibacterial activity test method in this study. The characterization of citronella essential oil revealed that it was light yellow in color, had the scent of citronella oil, a specific gravity of 0,879 g/mL, a refractive index of 1,471, and solubility in ethanol 80 at a ratio of 1:2. GC-MS analysis of the oil identified 20 compounds, with the main components being citronellal at 45,83%, geraniol at 5,88%, and citronellol at 15,55%. The antibacterial activity test of citronella essential oil against clinical isolates of S. mutans demonstrated inhibition zones of 9,43 mm; 11,8 mm; 13,3 mm; 14,6 mm; and 16,8 mm for concentrations of 2,5; 5; 10; 20; and 30%, respectively. These results indicate that citronella essential oil derived from Cymbopogon nardus L. has the potential to inhibit the growth of clinical isolates of S. mutans. Keyword: antibacterial activity, Streptococcus mutans clinical isolates, citronella essential oil, Cymbopogon nardus L.