Electronic Theses and Dissertation

IMUNOLOKALISASI RESEPTOR PROGESTERON PADA ENDOMETRIUM TIKUS PUTIH (Rattus norvegicus)
MODEL ENDOMETRITIS HASIL
INDUKSI BAKTERI

ABSTRAK
Endometritis merupakan peradangan pada endometrium yang disebabkan oleh mikroorganisme seperti Escherichia coli (E. coli) dan Staphylococcus aureus (S. aureus). Pada hewan yang menderita endometritis, corpus luteum (CL) terus menghasilkan hormon progesteron, namun hewan tersebut infertil. Penelitian ini bertujuan mengetahui ekspresi reseptor progesteron pada endometrium tikus putih (Rattus norvegicus) model endometritis hasil induksi bakteri yang dideteksi menggunakan teknik imunohistokimia (IHK). Hewan coba yang digunakan yaitu 21 ekor tikus putih betina dengan kriteria berat badan sekitar 200 g, dan kondisi fisik sehat secara klinis yang kemudian dibagi menjadi 7 kelompok (masing-masing n=3). Kelompok K0 (kontrol negatif) diberikan NaCl fisiologis sebanyak 0,1 ml; K1 diinduksi masing-masing dengan 50 l E. coli dosis 1,5 x 105 CFU/ml; K2 diinduksi S. aureus dosis 1,5 x 105 CFU/ml; K3 diinduksi dengan campuran bakteri E. coli dan S. aureus dosis 1,5 x 105 CFU/ml; K4 diinduksi E. coli dosis 1,5 x 108 CFU/ml; K5 diinduksi S. aureus dosis 1,5 x 108 CFU/ml; dan K6 diinduksi campuran bakteri E. coli dan S. aureus dosis 1,5 x 108 CFU/ml. Hewan coba diadaptasikan selama 2 minggu, lalu diinjeksi dengan progesteron selama 5 hari, setelah itu diinduksi dengan bakteri sesuai kelompok. Tiga puluh enam jam setelah perlakuan, tikus diterminasi untuk pengambilan organ uterus dan diproses secara histoteknik kemudian diwarnai dengan pewarnaan IHK metode avidin biotin complex peroxidase. Ekspresi reseptor progesteron pada endometrium diidentifikasi dengan metode intensitas skor (IS) dan data yang diperoleh dianalisis menggunakan uji Kruskal-Wallis dan uji lanjut Mann-Whitney U test. Hasil penelitian menunjukkan bahwa ekspresi reseptor progesteron pada endometrium uterus K0 berbeda nyata dengan K5 dan K6 (P0,05). Ekspresi reseptor progesteron pada endometrium uterus K1 dan K2 berbeda nyata dengan K5 dan K6 (P0,05). Kesimpulan penelitian ini adalah ekspresi reseptor progesteron mengalami penurunan pada tikus putih model endometritis yang diinjeksikan S. aureus 1,5 x 108 CFU/ml dan bakteri campuran E. coli 1,5 x 108 CFU/ml dan S. aureus 1,5 x 108 CFU/ml.

Kata kunci: Endometritis, reseptor progesteron, E. coli, S. aureus

IMMUNOLOCALIZATION OF PROGESTERONE RECEPTORS IN THE ENDOMETRIUM OF WHITE RAT (Rattus norvegicus) MODEL OF BACTERIAL-INDUCED ENDOMETRITIS ABSTRACT Endometritis is an inflammation of the endometrium caused by microorganisms such as Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). In animals suffering from endometritis, the corpus luteum (CL) continues to produce the hormone progesterone, but the animal is infertile. This study aims to determine the expression of progesterone receptors in the endometrium of white rats (Rattus norvegicus) model of bacterial induced endometritis detected using immunohistochemical techniques (IHK). The experimental animals used were 21 female white rats with body weight criteria of about 200 g, and clinically healthy physical conditions which were then divided into 7 groups (n = 3 each). Group K0 (negative control) was given physiological NaCl as much as 0,1 ml; K1 was induced respectively with 50 l E. coli at a dose of 1,5 x 105 CFU/ml; K2 was induced with S. aureus at a dose of 1,5 x 105 CFU/ml; K3 was induced with a mixture of bacteria E. coli and S. aureus at a dose of 1,5 x 105 CFU/ml; K4 induced with E. coli at a dose of 1,5 x 108 CFU/ml; K5 induced with S. aureus at a dose of 1,5 x 108 CFU/ml; and K6 induced with a mixture of E. coli and S. aureus at a dose of 1,5 x 108 CFU/ml. The experimental animals were adapted for 2 weeks, then injected with progesterone for 5 days, after which they were induced with bacteria according to the group. Thirty-six hours after treatment, the rats were terminated for uterine organ collection and histotechnically processed and then stained with IHK staining avidin biotin complex peroxidase method. Progesterone receptor expression in the endometrium was identified by intensity score (IS) method and the data obtained were analyzed using Kruskal-Wallis test and Mann-Whitney U test. The results showed that progesterone receptor expression in the uterine endometrium of K0 was significantly different from K5 and K6 (P0.05). Progesterone receptor expression in the uterine endometrium of K1 and K2 was significantly different from K5 and K6 (P0.05). The conclusion of this study is that progesterone receptor expression decreased in white rat endometritis model injected with S. aureus 1,5 x 108 CFU/ml and mixed bacteria E. coli 1,5 x 108 CFU/ml and S. aureus 1,5 x 108 CFU/ml. Keyword: Endometritis, progesterone receptors (PR), E. coli, S. aureus