Electronic Theses and Dissertation

Transplantasi ovarium pada uterus kelinci bunting semu dapat menyebabkan stres. Stres ini akan menyebabkan kerusakan pada uterus kelinci bunting semu yang diduga dapat dikurangi dengan pemberian ekstrak sarang semut. Penelitian ini bertujuan mengetahui perbandingan kemampuan ekstrak sarang semut dan kombinasi cyclosporine dan azathioprine dalam menurunkan kerusakan uterus kelinci New Zealand White (NZW) bunting semu yang mengalami transplantasi ovarium. Dalam penelitian ini digunakan enam ekor kelinci betina NZW bunting semu, berumur 3-5 tahun dan bobot badan 1,5-3,0 kg. Kelinci dikelompokkan dalam dua kelompok perlakuan (n=3), yaitu K1 dan K2. Seluruh kelinci
diinduksi bunting dengan injeksi 100 IU PMSG dan diikuti tiga hari kemudian dengan injeksi 75 IU hCG secara intramuskulus. Transplantasi ovarium dilakukan pada hari ke-8 (hari ke-0 adalah hari ketika injeksi hCG). Perlakuan pada semua kelompok dilakukan setiap pagi dan sore hari mulai pelaksanaan transplantasi ovarium sampai ovarium dikoleksi yang terdiri atas pemberian ekstrak tanaman sarang semut dengan dosis 200 mg per oral (K1) dan kombinasi cyclosporine 2,5 mg/kg secara intramuskulus dan azathioprine dosis 5 mg/kg bobot badan per oral (K2). Uterus dikoleksi lima hari setelah transplantasi lalu diproses secara histoteknik untuk pengamatan histopatologis uterus. Pada K1 dan K2 terdapat hiperemi, nekrosis pada epitel, dan infiltrasi sel radang. Meskipun jumlah nekrosis pada K1 lebih tinggi dibandingkan K2 (P

Ovarian transplantation in the uterus of pseudo pregnant rabbits can cause stress. This stress will cause damage to the uterus of pseudo pregnant rabbits which is thought to be reduced by giving anthill extract. This study aims to compare the ability of anthill extract and a combination of cyclosporine and azathioprine in reducing uterine damage to New Zealand White (NZW) pseudo pregnant rabbits who underwent ovarian transplantation. In this study, six female NZW pseudo-pregnant rabbits, 3-5 years old and 1.5-3.0 kg body weight, were used. Rabbits were grouped into two treatment groups (n=3), K1 and K2. All rabbits were induced pregnant by injection of 100 IU PMSG and followed three days later by injection of 75 IU hCG intramuscularly. Ovarian transplantation was performed on day 8 (day 0 was the day of hCG injection). Treatment in all groups was carried out every morning and evening from the implementation of ovarian transplantation until the ovaries were collected, consisting of the administration of anthill plant extract at a dose of 200 mg orally (K1) and a combination of cyclosporine 2.5 mg/kg intramuscularly and azathioprine at a dose of 5 mg/kg body weight orally (K2). Uterus was collected five days after transplantation and processed histotechnically for histopathological observation of the uterus. In K1 and K2 there was hyperemia, necrosis of the epithelium, and inflammatory cell infiltration. Although the amount of necrosis in K1 was higher than in K2 (P