Electronic Theses and Dissertation

Penggunaan ekstrak hipofisis dalam superovulasi memiliki beberapa keunggulan dibandingkan dengan hormon sintetik karena ekstrak hipofisis mengandung hormon gonadotropin seperti follicle stimulating hormone (FSH) dan luteinizing hormone (LH) yang berperan di dalam ovarium. Selain itu, di dalam ekstrak hipofisis juga terdapat growth hormone (GH) yang berperan dalam pertumbuhan fetus dan meningkatkan produksi susu induk. Tujuan penelitian ini adalah mengetahui potensi ekstrak hipofisis sapi (EHS) dalam menginduksi superovulasi berdasarkan peningkatan ekspresi estrogen receptor alpha (ERα) dan progesterone receptor-A (PR-A) pada jaringan uterus kelinci lokal. Dalam penelitian ini digunakan enam ekor kelinci betina lokal dengan kategori sudah pernah beranak dengan berat badan antara 1,8-2,2 kg dan satu ekor kelinci jantan lokal. Sebelum perlakuan, kelinci diadaptasikan selama 30 hari pada kandang yang terpisah dan diberi pakan serta air minum secara ad libitum. Setelah masa adaptasi selesai, kelinci dibagi atas dua kelompok perlakuan (n=3), yakni KL1 sebagai kelompok kontrol yang diinjeksi dengan NaCl fisiologis dengan dosis total 2,5 ml dan KL2 sebagai kelompok perlakuan yang diinjeksi dengan EHS dosis total sebanyak 2,6 ml. Injeksi NaCl fisiologis dan EHS dilakukan sebanyak lima kali dengan interval 12 jam. Injeksi pertama dilakukan pada pukul 20.00 WIB. Volume NaCl fisiologis pada KL1 dan EHS pada KL2 yang diinjeksikan sebanyak lima kali masing-masing adalah 1;0,5+0,5;0,3+0,3 ml (volume total 2,6 ml) per ekor kelinci. Dua belas jam setelah injeksi EHS terakhir, kelinci KL2 diinjeksi dengan 100 IU hCG dan dikawinkan dengan kelinci jantan, sedangkan kelinci KL1 dikawinkan tanpa pemberian hCG. Pada hari ke-6 setelah perlakuan, kelinci-kelinci pada kedua kelompok disembelih untuk pengambilan organ uterus yang diproses secara histoteknik dan diwarnai dengan pewarnaan imunohistokimia metode avidin biotin complex peroxidase (ABC methods). Data distribusi ERα dan PR-A pada jaringan uterus dianalisis secara deskriptif sedangkan perbedaan ekspresi kedua reseptor pada kedua kelompok kelinci berdasarkan skor intensitas dianalisis menggunakan uji Mann-Whitney U test. Berdasarkan hasil pengamatan ditemukan keberadaan ERα dan PR-A pada kelenjar uterus, lamina epithelia, dan lamina muskularis lapisan endometrium uterus kelinci. Ekspresi ERα pada uterus kelinci KL2 lebih tinggi dibandingkan KL1 (P

The use of pituitary extracts in superovulation has several advantages compared to synthetic hormones because pituitary extracts contain gonadotropin hormones such as follicle stimulating hormone (FSH) and luteinizing hormone (LH) which play a role in the ovaries. Apart from that, the pituitary extract also contains growth hormone (GH) which plays a role in fetal growth and increases milk production. The aim of this study was to determine the potential of bovine pituitary extract (EPS) in inducing superovulation based on the distribution and expression of estrogen receptor alpha (ERα) and progesterone receptor-A (PR-A) in local rabbit uterine tissue. In this study, 6 local female rabbits were used in the category of having given birth and weighing between 1.8-2.2 kg. Furthermore, one local male rabbit was also used. Before treatment, the rabbits were adapted for thirty days in individual cages.. After the adaptation period, the rabbits were divided into two treatment groups (n=3), namely KL1 as the control group which was injected with physiological NaCl with a total dose of 2.5 ml and KL2 as the treatment group which was injected with EPS with a total dose of 2.6 ml. Physiological NaCl and EPS injections were carried out five times at 12 hour intervals. The first injection was carried out at 20.00 WIB. The volume of physiological NaCl in KL1 and EPS in KL2 which was injected five times respectively was 1;0.5+0.5;0.3+0.3 ml (total volume 2.6 ml) per rabbit. Twelve hours after the last EPS injection, KL2 rabbits were injected with 100 IU hCG and mated with male rabbits, while KL1 rabbits were mated without hCG administration. On the 6th day after treatment, the rabbits in both groups were slaughtered to found the uterine organs which were processed histotechnically and stained with immunohistochemical staining using the avidin biotin complex peroxidase method (ABC methods). Data on the distribution of ERα and PR-A in uterine tissue were analyzed descriptively, while differences in the expression of the two receptors in the two groups of rabbits based on intensity scores were analyzed using the Mann-Whitney U test. Based on the results of observations, the presence of ERα and PR-A was found in the uterine glands, lamina epithelium, and lamina muscularis in the endometrial layer of the rabbit uterus. The ERα expression in the uterus of KL1 and KL2 rabbits was significantly different (P