Electronic Theses and Dissertation

Bromodosis adalah suatu keadaan dimana timbulnya bau kaki yang berlebih dari
kondisi normal. Permasalahan ini dapat mengurangi rasa percaya diri dan mengganggu
penampilan seseorang. Bromodosis disebabkan oleh bakteri diantaranya
Staphylococcus epidermidis dan Bacillus subtilis. Penelitian ini bertujuan untuk
memformulasikan sediaan foot spray dari minyak atsiri serai wangi dan mengukur
aktivitas antibakteri terhadap S. epidermidis dan B. subtilis. Hasil karakterisasi minyak
atsiri serai wangi berwarna kuning muda, beraroma khas serai wangi, indeks bias
1,4695, bobot jenis 0,879, dan 1:2 jernih dalam etanol 80%. Hasil analisis GC-MS
minyak atsiri serai wangi mengandung senyawa aktif tertinggi yakni sitronelal
48,80%, sitronelol 15,74%, dan geraniol 7,26%. Hasil uji aktivitas antibakteri minyak
atsiri serai wangi pada konsentrasi 6,25% menghasilkan zona hambat sebesar 12,26
mm terhadap S. epidermidis dan 11,38 mm terhadap B. subtilis. Hasil formulasi
sediaan foot spray di evaluasi dan didapatkan formula F0 tanpa minyak atsiri serai
wangi dan F1 minyak atsiri serai wangi 6,25% memenuhi syarat mutu semua
pengujian, sedangkan F2 minyak atsiri serai wangi 12,5% tidak memenuhi syarat mutu
pada pengujian organoleptik terjadi perubahan warna setelah cycling test. Hasil
pengujian hedonik F2 lebih banyak disukai panelis. Hasil uji aktivitas antibakteri
sediaan foot spray dari minyak atsiri serai wangi terbaik pada F2 dengan zona hambat
sebesar 14,86 mm terhadap S. epidermidis dan 12,53 mm terhadap B. subtilis.
Kesimpulan dari penelitian ini minyak atsiri serai wangi berpotensi sebagai
antibakteri, dapat diformulasikan dalam bentuk sediaan foot spray yang memenuhi
semua persyaratan mutu sediaan topikal yang baik pada F0 dan F1, namun F2 paling
banyak disukai panelis, serta sediaan foot spray dari minyak atsiri serai wangi
berpotensi sebagai antibakteri dan penghilang bromodosis.

Kata Kunci: Minyak atsiri serai wangi, Staphylococcus epidermidis, Bacillus subtilis,
Foot spray

Bromodosis is a condition where there is more than normal foot odor. This problem can reduce self-confidence and disrupt one's appearance. Bromodosis is caused by bacteria including Staphylococcus epidermidis and Bacillus subtilis. This study aims to formulate foot spray preparation from citronella essential oil and measure the antibacterial activity against S. epidermidis and B. subtilis. The characterization results of citronella essential oil are light yellow, typical citronella aroma, refractive index 1.4695, specific gravity 0.879, and 1:2 clear in 80% ethanol. The results of GC MS analysis of citronella essential oil contain the highest active compounds, namely citronellal 48.80%, citronellol 15.74%, and geraniol 7.26%. The results of the antibacterial activity test of citronella essential oil at concentration of 6.25% produced an inhibition zone of 12.26 mm against S. epidermidis and 11.38 mm against B. subtilis. The results of the formulation of foot spray were evaluated and found that formula F0 without citronella essential oil and F1 citronella essential oil 6.25% met the quality requirements of all tests, while F2 citronella essential oil 12.5% did not meet the quality requirements in organoleptic testing there was a color change after cycling test. The results of hedonic testing F2 were more favored by panelists. The results of antibacterial activity test of foot spray preparations from citronella essential oil are best in F2 with an inhibition zone of 14.86 mm against S. epidermidis and 12.53 mm against B. subtilis. The conclusion of this study is that citronella essential oil has potential as an antibacterial, can be formulated in the form of a foot spray that meet all the quality requirements of good topical preparations in F0 and F1, but F2 is most preferred by panelists, and foot spray preparations from citronella essential oil have potential as antibacterial and bromodosis remover. Keywords: Citronella essential oil, Staphylococcus epidermidis, Bacillus subtilis, Foot spray