Electronic Theses and Dissertation

Cymbopogon citratus atau sereh banyak digunakan dalam pengobatan tradisional untuk mengatasi penyakit seperti hipertensi, inflamasi dan diabetes melitus. Metabolit sekunder sereh yang paling umum adalah alkaloid, saponin, sterol, terpena, alkohol, keton, flavonoid, asam, yaitu asam klorogenat, kafeat, p-kumarat, dan gula. Liquid Chromatography Tandem-Mass Spectrometry (LC MS/MS) digunakan untuk menganalisis senyawa kompleks yang menawarkan sensitivitas dalam pengujiannya di berbagai aplikasi penelitian. Penelitian ini bertujuan untuk mengkarakterisasi senyawa kimia dalam ekstrak etanol Cymbopogon citratus diidentifikasi menggunakan instrumen LC MS/MS yang terbagi dalam dua mode ionisasi, yaitu ionisasi positif ([M+H]+) dan ionisasi negatif ([M-H]-). Data fragmentasi dianalisis menggunakan software MetFrag dan divalidasi oleh database lainnya, seperti MassBank, PubChem, ClassyFire sebagai penggolongan senyawa, KNApSAcK, dan KEGG. Hasil menunjukkan terdapat 86 senyawa yang memiliki final score > 0,9 yaitu sebanyak 33 senyawa dalam mode positif dan 53 senyawa dalam mode negatif. Golongan senyawa yang teridentifikasi meliputi flavonoid, karbohidrat, lipid, alkaloid, asam amino, asam karboksilat, antrakuinon, terpenoid, steroid, benzene/benzenoid, benzothiophene, furan, lignan, asam fenolat, asam keto, dan lakton. Konstituen utama yang dilaporkan adalah flavonoid, terutama senyawa flavon berupa orientin, luteolin, kaempferol 3-O-rhamnoside-7-O-glucoside, dan asam klorogenat yang berkontribusi pada aktivitas dari tanaman Cymbopogon citratus sebagai antioksidan dan antiinflamasi. Hasil penelitian ini memberikan informasi berharga tentang komposisi kimia dan potensi terapeutik ekstrak Cymbopogon citratus, khususnya untuk aplikasi farmasi dan pengembangan produk berbasis tanaman.

Cymbopogon citratus or lemongrass is widely used in traditional medicine to treat diseases such as hypertension, inflammation and diabetes mellitus. The most common secondary metabolites of lemongrass are alkaloids, saponins, sterols, terpenes, alcohols, ketones, flavonoids, acids, namely chlorogenic acid, caffeic acid, p-coumaric acid, and sugars. Liquid Chromatography Tandem-Mass Spectrometry (LC MS/MS) is used to analyze complex compounds that offer sensitivity in their testing in various research applications. This study aims to characterize chemical compounds in ethanol extract of Cymbopogon citratus identified using LC MS/MS instruments which are divided into two ionization modes, namely positive ionization ([M+H]+) and negative ionization ([M-H]-). Fragmentation data were analyzed using MetFrag software and validated by other databases, such as MassBank, PubChem, ClassyFire as compound classification, KNApSAcK, and KEGG. The results showed that there were 86 compounds that had a final score > 0.9, namely 33 compounds in positive mode and 53 compounds in negative mode. The identified compound groups include flavonoids, carbohydrates, lipids, alkaloids, amino acids, carboxylic acids, anthraquinones, terpenoids, steroids, benzene/benzenoids, benzothiophene, furans, lignans, phenolic acids, keto acids, and lactones. The main constituents reported were flavonoids, especially flavone compounds in the form of orientin, luteolin, kaempferol 3-O-rhamnoside-7-O-glucoside, and chlorogenic acid which contributed to the activity of the Cymbopogon citratus plant as an antioxidant and anti-inflammatory. The results of this study provide valuable information on the chemical composition and therapeutic potential of Cymbopogon citratus extract, especially for pharmaceutical applications and the development of plant-based products.