Electronic Theses and Dissertation

Testis merupakan organ reproduksi utama pada hewan jantan yang memiliki peranan penting dalam menghasilkan spermatozoa dan hormon testosteron. Penelitian ini bertujuan untuk mengetahui histologis dan histomorfometri testis ayam buras (Gallus gallus domesticus). Dalam penelitian ini digunakan organ testis bagian kanan dan kiri yang dikoleksi dari 3 ekor ayam buras berumur 6 bulan. Sampel difiksasi dalam larutan neutral buffer formalin 10% lalu diproses menjadi preparat histologi dan diwarnai dengan pewarnaan hematoksilin eosin (HE). Hasil histologis dianalisis secara deskriptif. Data histomorfometri dianalisis menggunakan uji t (independent sample t-test). Hasil penelitian menunjukkan bahwa histologis testis ayam buras terdiri dari tunika albuginea, tubulus seminiferus berisi sel-sel spermatogenik yang mengalami proses spermatogenesis dan sel Sertoli yang dikelilingi oleh jaringan interstisial. Rataan ketebalan tunika albuginea pada testis kiri dan kanan secara berturut-turut adalah 32,09±0,24 μm, 30,02±0,28 μm. Ketebalan epitel tubulus seminiferus pada testis kiri dan kanan secara berturut-turut adalah 82,52±0,29 μm, 80,07±0,60 μm. Diameter tubulus seminiferus pada testis kiri dan kanan secara berturut-turut adalah 182,48±0,3 μm 8, 180,17±0,49 μm. Diameter lumen tubulus seminiferus pada testis kiri dan kanan secara berturut-turut adalah 72,43±0,34 μm, 70,43±0,48 μm. Jarak antar tubulus seminiferus pada testis kiri dan kanan secara berturut-turut adalah 3,77±0,13 μm, 4,68±0,13 μm. Data histomorfometri yang telah dianalisis menunjukkan nilai signifikan (P

The testes are the main reproductive organ in male animals that have an important role in producing spermatozoa and the hormone testosterone. This study aims to determine the histology and histomorphometry of free-range chicken testes (Gallus gallus domesticus). This study used the right and left testicular organs collected from 3 6-month-old free-range chickens. Samples were fixed in 10% neutral buffer formalin solution and then processed into histological preparations and stained with hematoxylin eosin (HE) staining. Histological results were analyzed descriptively. Histomorphometric data were analyzed using independent sample t-test. The results showed that the histological testis of free-range chickens is wrapped by tunica albuginea and consists of seminiferous tubules containing spermatogenic cells that undergo the process of spermatogenesis and Sertoli cells surrounded by interstitial tissue. The mean thickness of tunica albuginea in left and right testes were 32.09±0.24 μm, 30.02±0.28 μm, respectively. The epithelial thickness of seminiferous tubules in left and right testes were 82.52±0.29 μm, 80.07±0.60 μm, respectively. The diameter of seminiferous tubules in left and right testes were 182.48±0.3 μm, 180.17±0.49 μm, respectively. The lumen diameter of seminiferous tubules in left and right testes were 72.43±0.34 μm, 70.43±0.48 μm, respectively. The distance between seminiferous tubules in the left and right testes were 3.77 ± 0.13 μm, 4.68 ± 0.13 μm, respectively. The histomorphometric data that has been analyzed shows a significant value (P