Electronic Theses and Dissertation

Konsentrasi hormon progesteron memiliki peran penting dalam mendukung kebuntingan dan perkembangan embrio pada sapi. Pengukuran progesteron secara non-invasif menggunakan sampel feses merupakan metode yang lebih ramah terhadap hewan dan dapat meminimalkan stres. Penelitian ini bertujuan untuk mengukur konsentrasi hormon progesteron secara non-invasif dari sampel feses sapi aceh bunting di trimester I, II, dan III. Penelitian ini menggunakan 30 ekor sapi aceh bunting. Sampel feses dikoleksi dari 10 sapi aceh bunting trimester I (umur kebuntingan 2-3 bulan), 10 sapi aceh bunting trimester II (umur kebuntingan 4-6 bulan), dan 10 sapi aceh bunting trimester III (umur kebuntingan 7-9 bulan). Analisis hormon dilakukan menggunakan metode enzyme-linked immunosorbent assay (ELISA). Data yang diperoleh dianalisis menggunakan uji Kruskal Wallis. Hasil penelitian menunjukkan rata-rata konsentrasi hormon progesteron pada sapi aceh bunting meningkat seiring bertambahnya umur kebuntingan, yaitu 1,14 ng/g pada trimester I, 2,91 ng/g pada trimester II, dan 3,25 ng/g pada trimester III. Analisis statistik menunjukkan adanya perbedaan yang signifikan (P

Progesterone plays a crucial role in supporting pregnancy and embryonic development in cattle. Measuring progesterone levels non-invasively through fecal samples offers an animal-friendly and stress-free method. This study aimed to evaluate progesterone levels non-invasively in pregnant aceh cattle during the first, second, and third trimesters. A total of 30 pregnant aceh cattle were included, with 10 cows in the first trimester (2–3 months of pregnancy), 10 in the second trimester (4–6 months), and 10 in the third trimester (7–9 months). Hormone analysis was conducted using the enzyme-linked immunosorbent assay (ELISA), and data were analyzed using the Kruskal-Wallis test. The results showed that progesterone levels in pregnant aceh cattle increased as pregnancy progressed, averaging 1.14 ng/g in the first trimester, 2.91 ng/g in the second trimester, and 3.25 ng/g in the third trimester. Statistical analysis confirmed a significant difference (P